ABSTRACT
The mortality of coronavirus disease 2019 (COVID-19) disease is very high among the elderly or individuals having comorbidities such as obesity, cardiovascular diseases, lung infections, hypertension, and/or diabetes. Our study characterizes the metagenomic features in severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2)-infected patients with or without type 2 diabetes, to identify the microbial interactions associated with its fatal consequences.This study compared the baseline nasopharyngeal microbiome of SARS-CoV-2-infected diabetic and nondiabetic patients with controls adjusted for age and gender. The metagenomics based on next-generation sequencing was performed using Ion GeneStudio S5 Series and the data were analyzed by the Vegan-package in R. All three groups possessed significant bacterial diversity and dissimilarity indexes (p < 0.05). Spearman's correlation coefficient network analysis illustrated 183 significant positive correlations and 13 negative correlations of pathogenic bacteria (r = 0.6-1.0, p < 0.05), and 109 positive correlations between normal flora and probiotic bacteria (r > 0.6, p < 0.05). The SARS-CoV-2 diabetic group exhibited a significant increase in pathogens and secondary infection-causing bacteria (p < 0.05) with a simultaneous decrease of normal flora (p < 0.05). The dysbiosis of the bacterial community might be linked with severe consequences of COVID-19-infected diabetic patients, although a few probiotic strains inhibited numerous pathogens in the same pathological niches. This study suggested that the promotion of normal flora and probiotics through dietary supplementation and excessive inflammation reduction by preventing secondary infections might lead to a better outcome for those comorbid patients.
ABSTRACT
The effects of COVID-19 are continuing to increase around the world as the pandemic claims thousands of lives. Bangladesh is no exception and has been greatly affected by SARS-CoV-2. Apart from the number of people who are or have been directly getting infected with this disease, millions of people are directly or indirectly facing many challenges to their livelihoods and the security of their food and nutritional supply, along with other societal issues created by the pandemic. In this study, a hybrid approach of online and telephone questionnaire surveys was used to investigate the food security of Dhaka city's inhabitants at household level. Approximately 80% of the respondents reported reduced income, and a quarter of respondents lost their jobs between March and June 2020. The frequency of fish consumption, an essential component of Bangladeshi diets, significantly reduced during the pandemic. This was especially apparent in affluent segments of the community. Out of the respondents, 75% reported an increase in the price of fish in Dhaka city. A range of coping strategies were observed: including decreasing the frequency of grocery shopping, shifting to online shopping, reducing consumption of high price commodities, reducing junk food consumption, cleaning fish and meat with hot water and vinegar, and increasing the consumption of protein and vitamin C rich food items. Prior to COVID-19, 80% of the households surveyed bought fish from wet markets. This number dropped to 45% during the pandemic. Many households substituted fish and meat with poultry, eggs and dried fish. About half of the households stockpiled rice, lentils and potatoes during the peak of the pandemic. However, if the pandemic lasts for a prolonged period, those living on low incomes in urban areas will experience some level of food insecurity from a reduced income or loss of work. Because of this, a large-scale sustainability policy should be undertaken to secure the food and nutritional security of low-income and middle-class household.